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Efficient regeneration and transformation systems for improving resistance to weevils in Ugandan sweetpotato cultivars
Sweetpotato is cultivated worldwide as a valuable source of food. Weevils cause 60-100% loss of productivity. The present study aims at genetically transforming selected Ugandan sweetpotato cultivars with genes that confer resistance to weevils. A tissue culture method is being optimised to be coupled with Agrobacterium transformation technique for regeneration of transgenic sweetpotato. Preliminary results show ability to form embryogenic callus in six tested cultivars. Magabali cultivar has shown best response of 72% embryogenic callus. Plant genotype, plant organ and concentration of growth hormone in media have shown significant effect on somatic embryogenesis and could affect plant regeneration efficiency.
 

Key Document Information

Authors: A. Sefasi, Gorettie Ssemakula, A. Kiggundu, S. Muukasa, E. Adipala, G. Tusiime, J.G.M. Majaliwa, Marc Ghislain

Subject: Weevil resistance through biotechnology

Publication Date: 2010

Publisher: RUFORUM

Contributors: Shiphar Mulumba

Format: pdf

Language: English

Keywords: Agrobacterium transformation, Ipomoea batatas, Plant regeneration, Weevil resistance

HOW TO CITE

Sefasi, A., Ssemakula, G., Ghislain, M., Kiggundu, A., Mukasa, S., Adipala, E., Tusiime, G. and Majaliwa, J.G.M., 2010. Efficient regeneration and transformation systems for improving resistance to weevils in Ugandan sweetpotato cultivars. In Second RUFORUM Biennial Regional Conference on" Building capacity for food security in Africa", Entebbe, Uganda, 20-24 September 2010 (pp. 525-529). RUFORUM.

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