Sweetpotato production is greatly constrained by two weevil species; Cylas puncticollis and C. brunneus that cause yield losses of up to 80%. The use of resistant sweetpotato cultivars is the most sustainable strategy to control the weevils. However, little success has been realized in developing resistant cultivars. Genetic engineering is a more viable option that offers a means to introduce resistant genes into sweetpotato. In this study the transgenic variety Jewel events with cry7Aa1, cry3Ca1, ET33- 34, cry7Aa1+cry3Ca1 and cry7Aa1+ ET33-34 will be used as source of resistance in transgenic breeding. The progenies will be selected for the presence of the Bt protein product and evaluated for weevil resistance and agronomic performance. The best performing progenies will be further used in the sweetpotato breeding programme to enhance resistance to the African weevils.
Subjects: Weevil resistance through biotechnology
Publication Date: 2010
HOW TO CITE
Rukarwa, R.J., Mukasa, S.B. and Kreuze, J.F., 2010. Enhancing sweetpotato resistance to African weevils (Cylas puncticollis and Cylas brunneus) through transgenic breeding. In Second RUFORUM Biennial Regional Conference on" Building capacity for food security in Africa", Entebbe, Uganda, 20-24 September 2010 (pp. 519-523). RUFORUM.