Extensive testing of LAMP (Loop Mediated Isothermal Amplification) has shown a high sensitivity and specificity in detecting sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), and begomoviruses comparable to using quantitative polymerase chain reaction (RT-QPCR). On-site detection of SPFMV, SPCSV, and begomoviruses was simple to perform in different geographical regions. LAMP assay has potential as a rapid diagnostic tool for SPFMV, SPCSV, and begomoviruses. At a current cost of $6.5 per assay, we anticipate the tool when used with bulked samples, which will be highly cost-competitive compared to biological, serological and molecular techniques.
Authors: Bramwel Wanjala, Jan Kreuze
Subjects: Crop protection, Sweetpotatoes, Sweetpotato agri-food systems
Publisher: International Potato Center
Publication Date: August 31, 2019
Rights: Open access: CC-BY-4.0
HOW TO CITE
International Potato Center. 2019. SASHA Brief 18. Rapid field based diagnostic tool for the detection of sweetpotato viruses is now a reality. Sweetpotato Action for Security and Health in Africa Project (SASHA). CIP. 2 p.