Extensive testing of LAMP (Loop Mediated Isothermal Amplification) has shown a high sensitivity and specificity in detecting sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), and begomoviruses comparable to using quantitative polymerase chain reaction (RT-QPCR). On-site detection of SPFMV, SPCSV, and begomoviruses was simple to perform in different geographical regions. LAMP assay has potential as a rapid diagnostic tool for SPFMV, SPCSV, and begomoviruses. At a current cost of $6.5 per assay, we anticipate the tool when used with bulked samples, which will be highly cost-competitive compared to biological, serological and molecular techniques.
Authors: Bramwel Wanjala, Jan Kreuze
Subjects: Crop protection, Sweetpotatoes, Sweetpotato agri-food systems
Publisher: International Potato Center
Publication Date: August 31, 2019
Rights: Open access: CC-BY-4.0
Keywords: 2019 SASHA Brief 18, Rapid diagnostic tool, Sweetpotato viruses
HOW TO CITE
International Potato Center. 2019. SASHA Brief 18. Rapid field based diagnostic tool for the detection of sweetpotato viruses is now a reality. Sweetpotato Action for Security and Health in Africa Project (SASHA). CIP. 2 p.