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Utility of thermotherapy and meristem tip for freeing sweetpotato from viral infection

Sweetpotato, (Ipomoea batatas L.), is vegetatively propagated crop. Due to the following cycles of propagation, viruses are accumulated, which contributes to the decline of sweetpotato yield and quality. Stem cuttings of Abees cultivar were collected from several locations in Egypt and kept under controlled greenhouse conditions. Samples were tested for the presence of sweetpotato feathery mottle virus (SPFMV) infection using dot-ELISA. Infected plants were thermotherapy treated by incubation of plants at 42°C/ day and 39°C/ night for 3 weeks followed by meristem tip culturing and allowed to grow in vitro. RT-PCR was carried out to confirm the success of SPFMV elimination. Tissue culture formed plants were tested routinely for successive 2 years using dot-ELISA. 0% infection was reported in the in vitro propagated plants. Sweetpotato plants were assisted the privatesector to enhance the final yield in their bio-farming system.

HOW TO CITE

El Far, M.M. and Ashoub, A., 2009. Utility of thermotherapy and meristem tip for freeing sweet potato from viral infection. Australian Journal of Basic and Applied Sciences, 3(1), pp.153-159.