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An efficient system of embryogenic suspension cultures and plant regeneration in sweetpotato

Sweetpotato is cultivated worldwide as a valuable source of food. Weevils cause 60-100% loss of productivity. The present study aims at genetically transforming selected Ugandan sweetpotato cultivars with genes that confer resistance to weevils. A tissue culture method is being optimised to be coupled with Agrobacterium transformation technique for regeneration of transgenic sweetpotato. Preliminary results show ability to form embryogenic callus in six tested cultivars. Magabali cultivar has shown best response of 72% embryogenic callus. Plant genotype, plant organ and concentration of growth hormone in media have shown significant effect on somatic embryogenesis and could affect plant regeneration efficiency.

Authors: Qingchang Liu, Hong Zhai, D.H. Lu, Y. Wang, Dapeng Zhang , Qingchang Liu, Hong Zhai, D.H. Lu, Y. Wang, Dapeng Zhang

Subjects: Embryogenic suspension cultures and plant regeneration in sweetpotato

Pages: 6

Publisher: International Potato Center (CIP)

Publication Date: 1997

Keywords: Embryogenic suspension cultures, Somatic embryogenesis, Tissue culture

HOW TO CITE

Liu, Q.C., Zhai, H., Lu, D.H., Wang, Y. and Zhang, D.P., 1997. An efficient system of embryogenic suspension cultures and plant regeneration in sweetpotato. Vitro Cellular and Developmental Biology–Plant, 1997, pp.265-270.