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Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives

Comparative analyses of genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives in Ipomoea series Batatas were conducted using amplified fragment length polymorphism (AFLP) and sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA. Low ITS divergence among thirteen species of ser. Batatas resulted in poorly resolved relationships. More variable AFLP characters were found to be more efficient in characterizing genetic diversity and phylogenetic relationships at both intra- and interspecific levels within ser. Batatas. Highly informative AFLP fingerprints of 36 accessions representing 10 species of ser. Batatas were generated using only six primer combinations. Of the species examined, I. trifida was found to be the most closely related to I. batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas. The highly polymorphic AFLP markers are a valuable tool in assessing genetic diversity and phylogenetic relationships of sweetpotato and its wild relatives.

Authors: Junchao Huang, Harold Corke, Mei Sun, Junchao Huang, Harold Corke, Mei Sun

Contributors: Shiphar Mulumba, Shiphar Mulumba

Subjects: Breeding

Pages:

Publisher: Genetic Resources and Crop Evolution

Publication Date: 2001

Keywords: AFLP, Ipomoea batatas, ITS, Phylogeny, Sweetpotato

HOW TO CITE

Huang, Junchao, Harold Corke, and Mei Sun. "Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives." Genetic Resources and Crop Evolution 49, no. 6 (2002): 541-550.