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Identifying a Randomly Amplified Polymorphic DNA (RAPD) Marker Linked to a Gene for Root-knot Nematode Resistance in Sweetpotato

The inheritance of resistance to root-knot nematode race 3 [Meloidogyne incognita (Kofoid & White) Chitwood] in sweetpotato [Ipomoea batatas (L.) Lam.] was studied in 71 progenies of the F1 single-cross population produced from the cross of resistant parent ‘Regal’ and susceptible parent ‘Vardaman’. The distribution frequency of the progenies based on log total nematode number (egg + juvenile counts) was a bimodal distribution with a ratio of 4 resistant : 1 susceptible. Based on this phenotypic ratio, the proposed genetic model was duplex polysomic inheritance (RRrrrr = resistant parent and rrrrrr = susceptible parent). Bulk segregant analysis in conjunction with the RAPD technique was used to identify a RAPD marker linked to a root-knot-nematode-resistance gene. Of 760 random decamer primers screened, 9 showed polymorphic bands between the two bulk DNA samples. Primer OP15(1500) produced a band in the resistant bulk but not in the susceptible bulk, suggesting a linkage in coupling phase. An estimated recombination fraction 0f 0.2421 +- 0.057 between the marker and the root-knot-nematode-resistance gene indicated linkage.

Authors: Kittipat Ukoskit, Paul Thompson, C.E. Watson, Jr., G.W. Lwrence, Kittipat Ukoskit, Paul Thompson, C.E. Watson, Jr., G.W. Lwrence

Contributors: Shiphar Mulumba, Shiphar Mulumba

Subjects: Breeding


Publisher: Journal of the American Society for Horticultural Science

Publication Date: 1997

Keywords: bulk segregant analysis, Ipomoea batatas, Meloidogyne incognita, Molecular marker, polyploid


Ukoskit, K., P. G. Thompson, C. E. Watson, and G. W. Lawrence. "Identifying a randomly amplified polymorphic DNA (RAPD) marker linked to a gene for root-knot nematode resistance in sweetpotato." Journal of the American Society for Horticultural Science 122, no. 6 (1997): 818-821.