This procedure covers the multiplication of in vitro sweetpotato germplasm for distribution and conservation purposes. The multiplication procedure is realized by a properly qualified technician (or auxiliary technician).This technique is based on the principle that appropriate culture conditions induce the growth of pre-existent terminal or axillar buds, resulting in a new plantlet. The nutritional and hormonal conditions of the medium break the bud’s latency and promote its rapid growth. Callus formation, followed by shoot regeneration, must be avoided because it can affect the genetic integrity of the clone. Following the protocol described below, after 4-5 weeks of in vitro culture plantlets may be conserved, multiplied again, or distributed to the national, international and CIP internal consignees.
Authors: International Potato Center , International Potato Center
Subjects: In vitro micropropagation of sweetpotato
Publisher: International Potato Center (CIP)
Publication Date: October 10, 2010