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Indicator plant diagnostic procedure – OP22

This OP explains how to use grafting – a technique whereby cut tissue surfaces of different plants are placed in close contact to effect a union. Grafting is considered to be a universal method for transmitting viruses, because grafting can transmit systemic viruses. Some grafts, however, are easier to do than others. Grafting is particularly useful for transmission of phloem-restricted viruses that cannot be transmitted mechanically and viruses whose vectors remain unknown, and for detecting viruses found in low concentrations. 

Authors: International Potato Center (CIP), International Potato Center (CIP)

Contributors: Margaret McEwan, Margaret McEwan

Subjects: Sweetpotato virus testing and indexing

Pages: 4

Publisher: International Potato Center (CIP)

Publication Date: February 11, 2010

Keywords: Grafting, Virus indexing, Virus testing