This manual is based on the experiences and lessons from the implementation of the Marando Bora project in Tanzania, which was led by International Potato Center (CIP). It shares the hands-on practices for the initiation and mass propagation of tissue culture plantlets, transportation, hardening and field establishment. This manual is intended for scientists and technicians who are working in limited resource settings to transfer sweetpotato tissue culture plantlets for field multiplication. It also provides seed system practitioners an overview of tissue culture based procedures required for germplasm management and exchange so as to ensure improvement of the quality of seed available to farmers.