In this study, a simple and efficient plant regeneration system in Egyptian sweetpotato Abees recalcitrant cultivar via indirect organogenesis was established. Two initiation medium and different regeneration medium were tested. Hormone free initiation medium (HFIM) treatment was found to be critical for induction of regenerative callus. Likewise, callus diagnostic structural histology confirmed de novo differentiation of meristematic domes in induced callus tissues and their further development into bud primordia. Shoot regeneration was dramatically improved by subculture of the initiated callus from HFIM onto different enriched cytokinin regeneration medium. Although, an overall analysis of variation also revealed a significant response for media used for shoot regeneration. The highest significant number of regenerated shoots per shooted callus (2.33) and frequency of regenerated shoots (80.90%) were obtained on benzyl adenine containing medium (BARM). Subsequently, regenerated shoots were rooted on hormone free medium (RM). Regenerated plants were acclimatized in controlled environment growth chamber and successfully established in the greenhouse. Hence, we report a reliable regeneration system for local recalcitrant cultivar Abees which could be used to exert selection pressure to abiotic stress or to transfer genes of agronomic interest and produce transgenic plants.
Publisher: Australian Journal of Basic and Applied Sciences
Publication Date: 2009
Identifier: ISSN 1991-8178
HOW TO CITE
El Far, M.M., El Mangoury, K. and Elazab, H.E.M. 2009. Novel Plant Regeneration for Egyptian Sweetpotato (Ipomoea Batatas (L.) Lam.) Abees Cultivar via Indirect Organogenesis Stimulated by Initiation Medium and Cytokinin Effects. Australian Journal of Basic and Applied Sciences, 3(2), pp.543-551.