To establish an efficient technique for sweetpotato (Ipomea batatas L) cv. Abees, an orange fleshed Egyptian cultivar, nodal segments excised from virus free plant material grown in an in vitro culture. Single node cuttings were cultured onto MS (Murashige and Skoog, 1962) established medium supplemented with 0.5 mg/l of IAA (3-Indoleacetic acid), NAA (1-Naphtalene acetic acid) or GA3 (Gibberellic acid) for one week. After that, nodal segment explants were transferred to MS medium supplemented with 20 or 60 g/l sucrose combined with or without 0.1 mg/l BA (6-Benzyladenine) The obtained shoots were rooted in hormonal-free MS-medium supplemented with 20 g/l sucrose. The optimum multiplication rate was obtained with segments formerly cultured on 0.5 mg/l GA3 and thereafter in 60 g/l sucrose. Each nodal segment gave rise to an average of one shoot with 8 nodes. The survival rate after acclimatization reached 95% and their growth was normal and healthy. Culturing of single-bud explant could produce 2 to 8 million plants following 9 subcultures. This was calculated on the basis of 20% losses of the obtained shoots in each subculture.
Authors: Mervat Ahmed, Mervat Ahmed
Subjects: In vitro micropropagation of sweetpotato
Publisher: African Potato Association
Publication Date: 2007
Keywords: Egypt, In vitro micropropagation, Multiplication rate, Survival rate, Tissue culture
HOW TO CITE
Mervat, M.M.E.L., 2007. OPTIMIZATION OF GROWTH CONDITIONS DURING SWEETPOTATO MICRO-PROPAGATION. In African Potato Association Conference Proceedings (Vol. 7, pp. 204-211).