The global meeting place for people interested in all things related to SWEETPOTATO

Share your research and experience, ask and answer questions, meet your peers.

EXPRESSION OF SWEET POTATO FEATHERY MOTTLE VIRUS COAT PROTEIN AND PRODUCTION OF SPECIFIC ANTISERUM FOR DIAGNOSTIC ASPECTS

Sweetpotato (Ipomoea batatas L.) has a diverse range of positive characteristics including high yield per unit area, nutritional value, and resistance to several production stresses. However, yield of sweetpotato is affected by several pathogens. One of these is the sweet potato feathery mottle virus (SPFMV), a major component of the sweet potato virus disease, SPVD combined with the sweet potato chlorotic stunt virus, SPCSV. SPFMV has long been recognized to occur wherever sweetpotatoes are grown. SPFMV, a type member of the potyviruses, is transmitted by several genera of aphids including the cotton aphid (Aphis gossypii) and the green peach aphid (Myzus persicae) in a non-persistent manner. Symptoms vary with regard to the cultivated variety and environmental factors that often makes identification difficult. Serological methods are widely used in the detection of viral infection.

 

Recently, advances in recombinant DNA technology, coupled with its ease to manipulate and its rapid growing rate in a less expensive media had established Escherichia coli (E. coli) as a leading host organism to produce high protein quantities of scientific interest. One of these interests is the expression of proteins as an antigen for antibody production.

Authors: Ahmed Ashoub, MERVAT M. M. EL-FAR, M. I. SALAMA, Ahmed Ashoub, MERVAT M. M. EL-FAR, M. I. SALAMA

Contributors: Segundo Fuentes, Segundo Fuentes

Subjects: Crop Management

Pages: 8

Publisher: Egyptian Journal of Genetics And Cytology

Publication Date: 2009

Keywords: Sweet potato, sweet potato feathery mottle virus (SPFMV)

HOW TO CITE

Ashoub, A., Mervat MM El-Far, and M. I. Salama. "Expression of Sweet potato feathery mottle virus coat protein and production of specific antiserum for diagnostic aspects." Egyptian Journal of Genetics And Cytology 38, no. 1 (2016).