Field experiments were conducted at three locations in Zambia to characterize 15 sweetpotato varieties for tuber yield and micro-nutrient content. Location and variety had significant effect on tuber yield. Marketable yield ranged from 5.22 – 9.69 t/ha with variety Kakamega recording the least yield. AMMI analysis also indicated significant effects of genotype and location for tuber yield. These accounted for 53 and 11% of the differences. The variety x location effect was significant for iron, but not zinc. Varieties Zambezi, K566632, 199062.1 and Mayai had high levels of β-carotene.