This technique is based on the principle that appropriate culture conditions induce the growth of a pre-existent bud, resulting in a new in vitro plantlet. The nutritional and hormonal conditions of the medium break the bud dormancy and promote its rapid growth. Callus formation, followed by plant regeneration, must be avoided because they can affect the genetic integrity of the clone. Following the protocol indicated below, after 4-5 weeks of sweetpotato in vitro culture, plantlets may be sub-cultured or transferred to soil in greenhouse.