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A genetic linkage map of sweetpotato [Ipomoea batatas (L.) Lam.] based on AFLP markers

Amplified Fragment Length Polymorphism (AFLP) based genetic linkage maps were developed for hexaploid sweetpotato (Ipomoea batatas (L.) Lam., 2n = 6x = 90) using a segregating population derived from a biparental cross between the cultivars ’Tanzania’ and ’Bikilamaliya’. A total of 632 (’Tanzania’) and 435 (’Bikilamaliya’) AFLPs could be ordered …
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Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA

Genetic diversity and relationships of 40 accessions of Ipomoea, representing ten species of series Batatas, were examined using ISSR markers and restriction-site variation in four non-coding regions of chloroplast DNA. A total of 2071 ISSR fragments were generated with 15 primers in these accessions and, on average, 52 bands per …
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Genetic Analysis of Sweetpotato and Wild Relatives using Inter-simple Sequence Repeats (ISSRs)

Inter-simple sequence repeat (ISSR) amplification was evaluates for its usefulness in generating DNA markers for sweetpotato and related wid species. ISSR markers were obtained through PCR amplification using simple sequence repeat (SSR) primers. Optimization of the reaction conditions was successfully achieved fro 24% out of 100 SSR primers screened. The …
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Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)

Background: Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed …
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Development of cleaved amplified polymorphic sequence (CAPS)-based markers for identification of sweetpotato cultivars

To develop a simple and reliable method to identify sweetpotato cultivars, we designed cleaved amplified polymorphic sequence (CAPS)-based markers and used them to perform genotyping of Japanese sweetpotato cultivars. In order to screen the CAPS-based markers, 13 primer pairs were designed from the exon sequences of 11 sweetpotato genes to …
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Autopolyploidy versus Allopolyploidy and Low-density Randomly Amplified Polymorphic DNA Linkage Maps of Sweetpotato

Low-density randomly amplified polymorphic DNA (RAPD) markers of sweetpotato [Ipomoea batatus (L.) Lam.; 2n = 6x = 90] were constructed from 76 pseudotestcross progenies obtained from ‘Vardaman’ x ‘Regal’. Of 460 primers, 84 generating 196 well-resolved repeatable markers were selected for genetic analysis. ‘Vardaman’ and ‘Regal’ testcross progenies were analyzed …
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Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives

Comparative analyses of genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives in Ipomoea series Batatas were conducted using amplified fragment length polymorphism (AFLP) and sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA. Low ITS divergence among thirteen species …
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Genotype * Environment Interactions for a Diverse Set of Sweetpotato Clones Evaluated across Varying Ecogeographic Conditions in Peru

Sweetpotato [Ipomoea batatas (L.) Lam.] is cultivated across a wide range of agrogeographical conditions. The objectives of this study were to analyze genotype * environment (G * E) interactions for sweetpotato yield (i.e., storage root yield, biomass, harvest index) and nutritional traits [i.e., root dry matter (RDM), starch (STA), and B-carotene (BCR) …
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Sweetpotato Viruses Cause Disease in Sweet Potato and Related Ipomoea spp.: Fulfilling Koch’s Postulates for a Divergent Group in the Genus Begomovirus

Sweet potato (Ipomoea batatas) and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae), known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch’s postulates have not been fullfilled for any of the viruses in …
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