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Tag Archives: dot-ELISA

Utility of thermotherapy and meristem tip for freeing sweetpotato from viral infection

Sweetpotato, (Ipomoea batatas L.), is vegetatively propagated crop. Due to the following cycles of propagation, viruses are accumulated, which contributes to the decline of sweetpotato yield and quality. Stem cuttings of Abees cultivar were collected from several locations in Egypt and kept under controlled greenhouse conditions. Samples were tested for …
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Comparison of methods to detect sweet potato feathery mottle virus (SPFMV) in sweet potatoes

Sensitive analytic techniques including double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA), direct ELISA, dot-ELISA and reverse transcription polymerase chain reaction (RT-PCR) are compared and evaluated for their capability to reliably distinguish between healthy and sweet potato feathery mottle virus (SPFMV) infected sweet potato plants. The DAS-ELISA is not adequate …
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Expression of sweetpotato feathery mottle virus coat protein and production of specific anti-serum for diagnostic aspects

Sweetpotato (Ipomoea batatas L.) has a diverse range of positive characteristics including high yield per unit area, nutritional value, and resistance to several production stresses. However, diagnostics aspects for detection of sweetpotato feathery mottle virus (SPFMV) are not well understood. This paper documents such aspect via expression of SPFMV coat …
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Biotechnology Applications for Improving Sweetpotato Production

Sweetpotato, (Ipomoea batatas L.), is a vegetatively propagated crop. Due to the cycles of propagation, viruses are accumulated, which contributes to the decline of sweetpotato yield and quality. Stem cuttings of Abees cultivar were collected from several locations in Egypt and kept under controlled greenhouse conditions. Samples were tested for …
  • Size: 110.6 kB
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  • Pages: 5

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