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Tag Archives: In vitro micropropagation

In vitro conservation of sweetpotato – OP26

urIn vitro sweetpotato germplasm conservation utilizes tissue culture techniques reducing plantlets growth. This is done applying incubation at low temperate. The methods described below allow the medium-term conservation of about 5,500 sweetpotato genotypes, following subculture procedures approximately every one year . 
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Micropropagation and hardening sweetpotato tissue culture plantlets

This manual is based on the experiences and lessons from the implementation of the Marando Bora project in Tanzania, which was led by International Potato Center (CIP). It shares the hands-on practices for the initiation and mass propagation of tissue culture plantlets, transportation, hardening and field establishment. This manual is …
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Optimization of growth conditions during sweetpotato micro-propagation

To establish an efficient technique for sweetpotato (Ipomea batatas L) cv. Abees, an orange fleshed Egyptian cultivar, nodal segments excised from virus free plant material grown in an in vitro culture. Single node cuttings were cultured onto MS (Murashige and Skoog, 1962) established medium supplemented with 0.5 mg/l of IAA …
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Tissue culture of Ipomoea batatas: Micropopagation and maintainance

Tissue culture allows the rapid clonal propagation of a large number of plantlets over a short period, as well as the maintenance of germplasm under controlled conditions in small spaces and with reduced labor requirements. This document describes the advantages, methodologies and materials used for tissue culture at the International …
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In vitro multiplication of sweetpotato – OP59

This technique is based on the principle that appropriate culture conditions induce the growth of a pre-existent bud, resulting in a new in vitro plantlet. The nutritional and hormonal conditions of the medium break the bud dormancy and promote its rapid growth. Callus formation, followed by plant regeneration, must be …
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In vitro multiplication of sweetpotato – OP59

 This procedure covers the multiplication of in vitro sweetpotato germplasm for distribution and conservation purposes. The multiplication procedure is realized by a properly qualified technician (or auxiliary technician).This technique is based on the principle that appropriate culture conditions induce the growth of pre-existent terminal or axillar buds, resulting in a …
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Introduction of sweetpotato to in vitro culture – OP58

The establishment of tuber crops into in vitro culture requires the isolation of vegetative structures (explants) containing buds. Explants should be inoculated in vessels containing a nutritive substrate (culture medium) where buds will grow to became whole plantlets. For the case of sweetpotato, the preferred explant for in vitro culture …
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Elimination of bacterial contaminants from sweetpotato in vitro cultures – OP53

Controlling internal or superficial bacterial contamination from sweet potato in vitro cultures can be difficult. Contaminants are not easy to detect visually, and most methods reported for bacteria elimination recommend the use of several antibiotics. Intensive use of these chemicals results toxic to the plants and frequently generates resistant strains. …
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Preparation of cultivation media – OP74

The use of an specific culture medium depends on the in vitro culture objective. In general, the media must contain a carbon source, minerals nutriments, vitamins, gelling agent (for semisolid media), growth regulators substances and other components as antioxidant substances for example. The preparation of culture medium is a critical …
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