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Tag Archives: NCM-ELISA

HQU – Sweetpotato NCM-ELISA Virus Detection – OP021

These operational procedures are for NCM-ELISA, an immuno-enzymatic test that uses nitrocellulose membranes instead of the polystyrene microtitration plates as a support for the reagents used in the serological reaction. The test is as sensitive as the direct double antibody sandwich ELISA (DAS-ELISA), easier to perform, and can be completed …
  • Size: 118.9 kB
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  • Pages: 20

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Viruses and virus-like diseases affecting sweet potato subsistence farming in southern Tanzania

A survey for sweet potato viruses was conducted in 32 farmer’s fields in Mbinga and Songea  districts of the Ruvuma region in Southern Tanzania. Plants showing virus-like symptoms were observed in 25  out of 32 fields examined. Sweet potato virus disease (SPVD) incidence was low in Mbinga district (16.7%) on …
  • Size: 542.5 kB
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  • Pages: 8

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Comparison of methods to detect sweet potato feathery mottle virus (SPFMV) in sweet potatoes

Sensitive analytic techniques including double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA), direct ELISA, dot-ELISA and reverse transcription polymerase chain reaction (RT-PCR) are compared and evaluated for their capability to reliably distinguish between healthy and sweet potato feathery mottle virus (SPFMV) infected sweet potato plants. The DAS-ELISA is not adequate …
  • Size: 168.0 kB
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  • Pages: 9

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Health status testing and virus elimination in sweetpotato – OP18

Virus elimination will be carried out in all materials maintained or generated at CIP, and prior to its distribution. The virus elimination technique is based on thermotherapy and meristem culture. Clones, which have tested negative to all known pathogens, using enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA) and the indicator …
  • Size: 22.8 kB
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  • Pages: 3

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NCM-ELISA detection of sweetpotato viruses – OP21

These operational procedures are for NCM-ELISA, an immuno-enzymatic test that uses nitrocellulose membranes instead of the polystyrene microtitration plates as a support for the reagents used in the serological reaction. The test is as sensitive as the direct double antibody sandwich ELISA (DAS-ELISA), easier to perform, and can be completed …
  • Size: 50.1 kB
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  • Pages: 5

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Survey and serological detection of sweet potato (Ipomoea batatas (L.) Lam.) viruses in Ethiopia

The study aimed to assess the recent magnitude of virus diseases attacking sweet potato in the main production areas of the country. Ninety seven sweet potato fields were visited in eastern and southern Ethiopia in 2009 and a total of 235 symptomatic and 735 asymptomatic vine cuttings were collected. The …
  • Size: 828.0 kB
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  • Pages: 11

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Health status testing and virus elimination in sweetpotato – OP18

Virus elimination will be carried out in all materials maintained or generated at CIP, and prior to its distribution. The virus elimination technique is based on thermotherapy and meristem culture. Clones, which have tested negative to all known pathogens, using enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA) and the indicator …
  • Size: 33.4 kB
  • Type:
  • Pages: 6

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