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Tag Archives: Tissue culture

A low-cost medium for sweetpotato micropropagation

Tissue culture has not been optimised in sweet potato (Ipomoea batatas (L) Lam) propagation due to high costs. With an aim of producing affordable disease-free seedlings, a low cost medium was developed and used to regenerate two sweet potato varieties (KEMB 36 and Tainung). The conventional sources of MS nutrients were substituted …
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In vitro conservation of sweetpotato – OP26

urIn vitro sweetpotato germplasm conservation utilizes tissue culture techniques reducing plantlets growth. This is done applying incubation at low temperate. The methods described below allow the medium-term conservation of about 5,500 sweetpotato genotypes, following subculture procedures approximately every one year . 
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Micropropagation and hardening sweetpotato tissue culture plantlets

This manual is based on the experiences and lessons from the implementation of the Marando Bora project in Tanzania, which was led by International Potato Center (CIP). It shares the hands-on practices for the initiation and mass propagation of tissue culture plantlets, transportation, hardening and field establishment. This manual is …
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Optimization of growth conditions during sweetpotato micro-propagation

To establish an efficient technique for sweetpotato (Ipomea batatas L) cv. Abees, an orange fleshed Egyptian cultivar, nodal segments excised from virus free plant material grown in an in vitro culture. Single node cuttings were cultured onto MS (Murashige and Skoog, 1962) established medium supplemented with 0.5 mg/l of IAA …
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  • Pages: 9

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Tissue culture of Ipomoea batatas: Micropopagation and maintainance

Tissue culture allows the rapid clonal propagation of a large number of plantlets over a short period, as well as the maintenance of germplasm under controlled conditions in small spaces and with reduced labor requirements. This document describes the advantages, methodologies and materials used for tissue culture at the International …
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In vitro conservation of sweetpotato – OP26

Operational procedures on in vitro sweetpotato germplasm conservation, which  utilizes tissue culture techniques reducing plantlets growth. This is done applying incubation at low temperature. The methods described allow the medium-term conservation of about 5,500 sweetpotato genotypes, following subculture procedures approximately every one year.
  • Size: 28.4 kB
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  • Pages: 5

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In vitro multiplication of sweetpotato – OP59

This technique is based on the principle that appropriate culture conditions induce the growth of a pre-existent bud, resulting in a new in vitro plantlet. The nutritional and hormonal conditions of the medium break the bud dormancy and promote its rapid growth. Callus formation, followed by plant regeneration, must be …
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An efficient system of embryogenic suspension cultures and plant regeneration in sweetpotato

Sweetpotato is cultivated worldwide as a valuable source of food. Weevils cause 60-100% loss of productivity. The present study aims at genetically transforming selected Ugandan sweetpotato cultivars with genes that confer resistance to weevils. A tissue culture method is being optimised to be coupled with Agrobacterium transformation technique for regeneration …
  • Size: 148.7 kB
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  • Pages: 6

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