Tag Archives: Tissue culture

 This procedure covers the multiplication of in vitro sweetpotato germplasm for distribution and conservation purposes. The multiplication procedure is realized by a properly qualified technician (or auxiliary technician).This technique is based on the principle that appropriate culture conditions induce the growth of pre-existent terminal or axillar buds, resulting in a …

The establishment of tuber crops into in vitro culture requires the isolation of vegetative structures (explants) containing buds. Explants should be inoculated in vessels containing a nutritive substrate (culture medium) where buds will grow to became whole plantlets. For the case of sweetpotato, the preferred explant for in vitro culture …

The use of an specific culture medium depends on the in vitro culture objective. In general, the media must contain a carbon source, minerals nutriments, vitamins, gelling agent (for semisolid media), growth regulators substances and other components as antioxidant substances for example. The preparation of culture medium is a critical …

Tissue culture materials consist of small aseptic plantlets growing on a synthetic nutrient medium. The aseptic nature of this material makes it an ideal method for international exchange of germplasm as it minimizes the risk of transmitting fungal and bacterial diseases. This document contains information for the recipient of in …

The establishment of tuber crops into in vitro culture requires the isolation of vegetative structures (explants) containing buds. Explants should be inoculated in vessels containing a nutritive substrate (culture medium) where buds will grow to became whole plantlets. For the case of sweetpotato, the preferred explant for in vitro culture …