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Tag Archives: Virus indexing

HQU – Sweetpotato Indicator plant diagnostic procedure – OP022

Author: International Potato Center Contributors: Segundo Fuentes November 13, 2012

This OP explains how to use grafting – a technique whereby cut tissue surfaces of different plants are placed in close contact to effect a union. Grafting is considered to be a universal method for transmitting viruses, because grafting can transmit systemic viruses. Some grafts, however, are easier to do …
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  • Pages: 15

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HQU – Sweet Potato Virus Indexing Procedure – OP023

Author: International Potato Center Contributors: Segundo Fuentes November 13, 2012

This standard procedure has been established based on current information for virus testing of sweetpotato germplasm, with the recognition that it will need to be revised as new information becomes available. The procedure includes symptomatology in sweetpotato plants grown in greenhouse (pots), as well as in Ipomoea setosa plants grafted …
  • Size: 262.8 kB
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  • Pages: 18

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Germplasm Virus Indexing: Supporting ISO/IEC 17025 Accreditation

Germplasm Virus Indexing

Author: J. Arellano, A. Perez et al. Contributors: Segundo Fuentes April 01, 2012

This presentation has information on Germplasm Virus Indexing: ISO/IEC 17025 accreditation, Virus indexing procedure, Analysis of indexing results, Research derived from indexing, Maintaining ISO accreditation and New molecular tests (validation). most of the findings are supported with beautiful images illustrating the study process. CIP was awarded the ISO/IEC 17025 accreditation on February 2008 and applies this ISO …
  • Size: 8.1 MB
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  • Pages: 80

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Principal Virus Diseases of Sweetpotato-Their Control and Eradication

Author: James Moyer Contributors: Segundo Fuentes, Administrator 1988

This article by Moyer describes the principal virus diseases, control and eradication in sweetpotato. One major proposed solution to the problem of viruses in sweetpotato germplasm will include more research on etiology of these diseases. This can be effective and reliable for virus-indexing or detection procedure development.
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Sweetpotato virus indexing procedure – OP23

Author: International Potato Center Contributors: Segundo Fuentes, Administrator, International Potato Center February 02, 2010

Virus indexing combines knowledge of viruses with methodologies for their detection to assure the effective safe movement of sweetpotato germplasm. Due to low virus titers and the absence of symptoms from single infections in sweetpotato by most viruses, grafting onto indicator plants is often required to boost titers and detect …
  • Size: 74.1 kB
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  • Pages: 4

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Indicator plant diagnostic procedure – OP22

Author: International Potato Center Contributors: Segundo Fuentes, Administrator, International Potato Center , Margaret McEwan February 11, 2010

This OP explains how to use grafting – a technique whereby cut tissue surfaces of different plants are placed in close contact to effect a union. Grafting is considered to be a universal method for transmitting viruses, because grafting can transmit systemic viruses. Some grafts, however, are easier to do …
  • Size: 31.7 kB
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  • Pages: 4

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NCM-ELISA detection of sweetpotato viruses – OP21

Author: International Potato Center Contributors: Segundo Fuentes, Administrator, International Potato Center , Margaret McEwan February 02, 2012

These operational procedures are for NCM-ELISA, an immuno-enzymatic test that uses nitrocellulose membranes instead of the polystyrene microtitration plates as a support for the reagents used in the serological reaction. The test is as sensitive as the direct double antibody sandwich ELISA (DAS-ELISA), easier to perform, and can be completed …
  • Size: 50.1 kB
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  • Pages: 5

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