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Tag Archives: Virus testing

SASHA Brief 2015: Improving Sweetpotato Virus Diagnostics

Author: Jan Kreuze Contributors: Jan Kreuze 2015

This flyer describes progress made between June 2014 and July 2015 to develop an easily accessible distribution map of all relevant viruses and virus strains infecting sweetpotato throughout SSA as well as appropriate diagnostic methods and protocols to detect them. It provides details about a generic virus detection method developed …
  • Size: 515.6 kB
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  • Pages: 2

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Determining the Pan-African sweetpotato virome: understanding virus diversity, distribution and evolution and their impacts on sweetpotato production in Africa

virus genome

Author: Dina Gutierrez, Martine Zandjanakou-Tachin et al. Contributors: Jan Kreuze, Segundo Fuentes 2012

Food security remains a huge challenge for the millions of Africans dependent on agriculture for their subsistence. A low-level agricultural productivity and a high percentage of poor and undernourished people are common in Africa, particularly in sub-Saharan Africa (SSA). Sweetpotato, Ipomoea batatas (L.) Lam. (Family Convolvulaceae), is among the most …
  • Size: 1.6 MB
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  • Pages: 1

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HQU – Sweetpotato NCM-ELISA Virus Detection – OP021

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes November 03, 2012

These operational procedures are for NCM-ELISA, an immuno-enzymatic test that uses nitrocellulose membranes instead of the polystyrene microtitration plates as a support for the reagents used in the serological reaction. The test is as sensitive as the direct double antibody sandwich ELISA (DAS-ELISA), easier to perform, and can be completed …
  • Size: 118.9 kB
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  • Pages: 20

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HQU – Sweetpotato Indicator plant diagnostic procedure – OP022

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes November 13, 2012

This OP explains how to use grafting – a technique whereby cut tissue surfaces of different plants are placed in close contact to effect a union. Grafting is considered to be a universal method for transmitting viruses, because grafting can transmit systemic viruses. Some grafts, however, are easier to do …
  • Size: 60.2 kB
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  • Pages: 15

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HQU – Sweet Potato Virus Indexing Procedure – OP023

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes November 13, 2012

This standard procedure has been established based on current information for virus testing of sweetpotato germplasm, with the recognition that it will need to be revised as new information becomes available. The procedure includes symptomatology in sweetpotato plants grown in greenhouse (pots), as well as in Ipomoea setosa plants grafted …
  • Size: 262.8 kB
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  • Pages: 18

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Health status testing and virus elimination in sweetpotato – OP18

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes, Margaret McEwan February 16, 2010

Virus elimination will be carried out in all materials maintained or generated at CIP, and prior to its distribution. The virus elimination technique is based on thermotherapy and meristem culture. Clones, which have tested negative to all known pathogens, using enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA) and the indicator …
  • Size: 22.8 kB
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  • Pages: 3

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Indicator plant diagnostic procedure – OP22

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes, Margaret McEwan February 11, 2010

This OP explains how to use grafting – a technique whereby cut tissue surfaces of different plants are placed in close contact to effect a union. Grafting is considered to be a universal method for transmitting viruses, because grafting can transmit systemic viruses. Some grafts, however, are easier to do …
  • Size: 31.7 kB
  • Type:
  • Pages: 4

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NCM-ELISA detection of sweetpotato viruses – OP21

Author: International Potato Center Contributors: Jan Kreuze, Segundo Fuentes, Margaret McEwan February 02, 2012

These operational procedures are for NCM-ELISA, an immuno-enzymatic test that uses nitrocellulose membranes instead of the polystyrene microtitration plates as a support for the reagents used in the serological reaction. The test is as sensitive as the direct double antibody sandwich ELISA (DAS-ELISA), easier to perform, and can be completed …
  • Size: 50.1 kB
  • Type:
  • Pages: 5

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